Journal: bioRxiv
Article Title: Rapid, automatic typing of Clostridioides difficile Ribotypes Using MALDI-TOF MS
doi: 10.1101/2024.01.24.576988
Figure Lengend Snippet: Proposed laboratory workflow with the implementation of Clostridioides difficie typing by MALDI-TOF MS methodology presented in this paper. The workflow shows a GeneXpert-dependent option and a direct culture option for those laboratories with no GeneXpert system available. PFGE: Pulsed-Field Gel Electrophoresis. WGS: Whole-Genome Sequencing.
Article Snippet: Before analysis, all isolates were re-identified using MALDI-TOF MS technology in an MBT Smart MALDI Biotyper (Bruker Daltonics, Bremen, Germany) with the updated database containing 11,096 mass spectra profiles, applying the standard protocol for rapid, on-plate protein extraction with 100% formic acid followed by HCCA (α-Cyano-4-hydroxycinnamic acid) matrix solution.
Techniques: Pulsed-Field Gel, Electrophoresis, Sequencing